| |
Percentage expressing cells
|
Mean fluorescence
|
|---|
|
Mutant
|
WT (n)
|
Heterozygote (n)
|
Homozygote (n)
|
WT (n)
|
Heterozygote (n)
|
Homozygote (n)
|
|---|
|
MommeD7
|
55 ± 7 (7)
|
80 ± 3* (13)
|
NA
|
286 ± 43 (7)
|
700 ± 52* (13)
|
NA
|
|
MommeD8
|
54 ± 4 (40)
|
41 ± 4* (55)
|
15 ± 4* (15)
|
287 ± 25 (40)
|
244 ± 28* (55)
|
161 ± 27* (15)
|
|
MommeD9
|
59 ± 4 (8)
|
39 ± 6* (13)
|
NA
|
326 ± 37 (8)
|
228 ± 27* (13)
|
NA
|
|
MommeD10
|
55 ± 4 (46)
|
44 ± 3* (87)
|
22 ± 2* (6)
|
295 ± 33 (46)
|
269 ± 26* (87)
|
243 ± 19* (6)
|
- The percentage of expressing cells was determined by using a GFP+ gate, which was set to exclude 99.9% of wild-type (WT) cells. Data were collected from at least four litters in each case. The data for the MommeD9 colony were collected using a different laser. Each mutant line has a significantly different expression profile to that of wild-type littermates, reproducible over many generations. *p ≤ 0.0001.
