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Table 1 Quantitative analysis of GFP expression following heterozygous intercrosses

From: A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development

  Percentage expressing cells Mean fluorescence
Mutant WT (n) Heterozygote (n) Homozygote (n) WT (n) Heterozygote (n) Homozygote (n)
MommeD7 55 ± 7 (7) 80 ± 3* (13) NA 286 ± 43 (7) 700 ± 52* (13) NA
MommeD8 54 ± 4 (40) 41 ± 4* (55) 15 ± 4* (15) 287 ± 25 (40) 244 ± 28* (55) 161 ± 27* (15)
MommeD9 59 ± 4 (8) 39 ± 6* (13) NA 326 ± 37 (8) 228 ± 27* (13) NA
MommeD10 55 ± 4 (46) 44 ± 3* (87) 22 ± 2* (6) 295 ± 33 (46) 269 ± 26* (87) 243 ± 19* (6)
  1. The percentage of expressing cells was determined by using a GFP+ gate, which was set to exclude 99.9% of wild-type (WT) cells. Data were collected from at least four litters in each case. The data for the MommeD9 colony were collected using a different laser. Each mutant line has a significantly different expression profile to that of wild-type littermates, reproducible over many generations. *p ≤ 0.0001.