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Figure 7 | Genome Biology

Figure 7

From: A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development

Figure 7

Bisulfite analysis of the HS-40 enhancer element. (a) DNA was extracted from the spleen of 4-week-old wild-type (FVB/NJ; three mice), MommeD5-/+ (three mice), MommeD10-/+ (three mice) mice and from the tail of MommeD10-/- mice (two mice). After bisulfite sequencing, the percentage of CpG methylation at each CpG site in the cloned region was analyzed, and averaged across individuals of the same genotype. None are significantly different from the wild-type mouse. Error bars represent SEM. (b) DNA was extracted from the tail of 4-week-old wild-type (C57BL/6; four mice) and Dnmt3b+/- (four mice) mice. After bisulfite sequencing, the percentage of CpG methylation at each CpG site in the cloned region was analyzed, and averaged across individuals of the same genotype. The Dnmt3b+/- mice have significantly (p < 0.001) less methylation than the wild-type mice. Error bars represent SEM.

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