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Figure 5 | Genome Biology

Figure 5

From: A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development

Figure 5

A mutation in Hdac1 correlates with the MommeD5-/- phenotype. (a) A 7 bp deletion was detected in exon 13 of Hdac1. Representative chromatograms from the wild-type (WT) allele, the mutant allele, and one heterozygote are shown. This deletion alters the reading frame, changing the last 12 amino acids and adding 25 extra amino acids. (b) Whole-cell lysates from six individual 10.5 dpc MommeD5+/+ and MommeD5-/+ embryos, and six pooled MommeD5-/- embryos were probed with antibodies to the Hdac1 carboxyl terminus (top panel), Hdac2 (top panel) and Hdac1 amino terminus (bottom panel). Anti-γ-tubulin was used as a loading control in each case. Quantification of the Hdac1 carboxyl terminus relative to γ-tubulin shows negligible binding in MommeD5-/- mice. Quantification of Hdac2 levels relative to γ-tubulin shows increased Hdac2 in MommeD5-/+ and MommeD5-/- embryos. Quantification of Hdac1 amino terminus relative to γ-tubulin shows equal levels of Hdac1 in all mice. A peptide blocking experiment was performed to confirm band identity. A representative western blot is shown. Error bars represent SEM. (c) A representative litter from a MommeD5-/+ intercross at 10.5 dpc. MommeD5-/- embryos (bottom right) are always dramatically smaller than MommeD5+/+ and MommeD5-/+ littermates.

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