Figure 3

Hematopoietic abnormalities in MommeD7 mice. (a) Examples of phenotypically normal and abnormal (pale) embryos at 17.5 dpc. Abnormal embryos are assumed to be homozygous for MommeD7. Scale bar = 5 mm. (b) Spleen weights from MommeD7^+/+ and MommeD7^-/+ adult mice. Error bars represent SEM. ***p < 0.0001. (c) Blood smears from MommeD7^+/+ (left) and MommeD7^-/+ (right) mice stained for reticulocytes (shown with arrowheads). (d) Representative histograms showing propidium iodide fluorescence in MommeD7^+/+ (left) and MommeD7^-/+ (right) mice. In each case 10,000 reticulocytes were counted. Red blood cells (RBC), reticulocytes (RET) and nucleated cells (NC) are shown. (e) Histogram showing relative levels of GFP fluorescence in red blood cells and reticulocytes. Averages were calculated from three wild-type and three heterozygous mice. **p < 0.005; error bars represent SEM.