Figure 5

Ras and TGFβ exhibit positive reciprocal regulation in mammary epithelial cells. (a) Immunofluorescence showing Smad4 nuclear translocation following short-term Ras expression in the mammary gland. Nuclei (blue), Smad4 (green), cytokeratin 8 (red). (b) Western blot analysis demonstrating phosphorylation of Smad1/3 after 96 h of Ras activation in vivo. (c) Quantification of western analysis. (d) Western analysis showing activated, GTP-bound Ras in NMuMG cells following TGFβ treatment. (e) Western analysis showing activated MEK in NMuMG cells following TGFβ treatment.