Figure 5

Expression profiles comparing relative mRNA and protein abundance. The panels summarize mRNA and protein abundance data for individual P. falciparum genes, with PlasmoDB accession numbers indicated below the gene names. Both the relative mRNA and protein abundance levels determined in this study for four schizont-stage time points (TPs) are based on the same parasite samples from a single large-scale in vitro culture and are indicated by gray and colored lines (see panel legends), respectively. In addition, the previously published transcript dynamics across the whole intra-erythrocytic life cycle [2] are included as black lines, with the black dots representing the corresponding raw data. All abundance profiles were mean-centered around zero based on the data points between 34 and 46 hours post-invasion. Asterisks in the panel legends denote cases for which analysis of variance (ANOVA) P < 0.01 and maximum fold change >1.4×. The insert in the eukaryotic initiation factor (eIF)5A panel depicts actual two-dimensional gel images to provide an approximate impression of absolute protein abundance levels between the three eIF5A isoforms.