Representative two-dimensional DIGE gels of P. falciparum schizont-stage proteins. (a) Protein reference pool (internal standard) labeled with Cy2. (b) Overlay of images showing Cy3-labeled and Cy5-labeled parasite proteins from time point (TP) samples 1 (TP1, green) and 3 (TP3, red). Proteins were separated in the first dimension along a nonlinear pH gradient (pH3-7NL, 24 cm Immobiline DryStrip [GE Healthcare]), and in the second dimension on an 11% polyacrylamide gel. Proteins/protein isoforms identified by tandem mass spectrometry are highlighted in color. In instances where more than one spot was identified as the same protein, the spots were numbered in numerical order from left to right (not shown), except for enolase, for which spot numbers are denoted in the figure. The molecular weight marker is indicated in kDa. DIGE, differential gel electrophoresis.