FISH examples. (a) Examples of FISH experiments using species-specific BAC clones yielding duplicated signals around the centromere. The CH250 and CH271 are BAC libraries specific for macaque and gibbon, respectively. The DAPI-stained chromosome without the signal is reported on the left to better show the morphology of the chromosome. (b) FISH experiment using the BAC clone CH250-417O7 (MMU2) on a macaque metaphase, showing pericentromeric signals on several chromosomes.