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Figure 5 | Genome Biology

Figure 5

From: Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment

Figure 5

Genetic interactions support the proteomic observations. (a) Scatter plots of correlation coefficients between genetic profiles for the two Set3C subunits, Set3 and Hos2, in S. cerevisiae and S. pombe across 239 direct homologs between the two species. In both species Sif2 (Hif2) is highly correlated with both Set3 and Hos2, consistent with its being a subunit of the core Set3C, whilst Hst1 (part of the Sc_Set3C extension) is uncorrelated with either Set3 or Hos2. Pho23 (Png2), a subunit of Rpd3C(L), correlates better with Set3 and Hos2 than does Rco1 (Cph1), a subunit of Rpd3C(S). The 90th percentile of the data is indicated. (b) Subunits of the core Set3C and Set3C extension show different genetic interaction patterns. Shown are genetic interactions between Set3C core and extension subunits and Swr1C, SAGA and Prefoldin subunits. Color-coding of the interaction magnitude (shown in the key) is as follows: shades of cyan indicate synthetic sick/lethal (negative) interactions typically observed between genes acting on parallel pathways; shades of yellow represent suppressive (positive) interactions seen primarily between genes acting on the same pathway and within stable protein complexes. (c) Msc1 in S. pombe is a member of Swr1C, while its S. cerevisiae ortholog (Ecm5) is not. Genetic profiles of members of the complex in the two species are shown with Msc1 and Ecm5 profiles aligned at the bottom. Genetic pattern of Msc1 is very similar to the rest of the complex and positive genetic interactions with the other members indicate that it is a bona fide member of Swr1C in S. pombe. Color-coding is as for (b). (d) A scatter plot of pair-wise correlation coefficients of genetic profiles of members of Swr1C in S. cerevisiae and S. pombe. Consistent with (c), data-points corresponding to pairs containing Msc1 or Ecm5 form an outlier group and are strongly correlated in S. pombe, but not in S. cerevisiae.

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