Figure 3

Validation of ChIP-on-chip results. (a) ChIP products for M12 cells treated with UV-C or untreated controls (control) were analyzed by qRT-PCR. The data are expressed as relative fold change of real-time PCR results to untreated control ChIP sample. The experiments were performed in triplicates and the error bars represent the triplicate data. (b) Western blot analysis of Egr2 (an Egr1 target gene) to define time of maximum induction. (c) M12 cells were treated with siRNA against Egr1 (SiEgr1) or SiGenome control (SiControl) for 48 h followed by UV-C irradiation and western blot confirmed the suppression of Egr1 in SiEgr1 treated cells. (d) qRT-PCR analysis of Egr1 target genes using RNA extracted from SiEgr1- or SiGenome control-treated M12 cells with or without UV-C irradiation. All the results are expressed relative to GAPDH.