Figure 2
ChIP-on-chip hybridization results. (a) Western analysis of ChIP products for M12 cells treated with UV-C or not (control). (b) Yield and size of DNA precipitated with anti-Egr1 from M12 cells following treatment with UV-C. (c) Distribution of the consensus Egr1-binding sequence (5' GCGGGGGCG 3') in approximately 17,000 human genes. (d) M-A plots of promoter array hybridization intensities of ChIP products from control M12 cells and cells treated with 40 J/m2 UV-C for 2 h. The lower panel shows significance (volcano) plots of the hybridization intensity data for ChIP products of M12 cells treated with UV-C for 2 h compared with control non-treated ChIP sample. The right arm of the lower plot shows significantly bound promoters.