Figure 1
Egr1 induction upon UV-C stimulation. (a) Time course and dose response of Egr1 induction upon UV-C stimulation was measured by qRT-PCR. RNA was collected at various time points and at different doses of UV-C in order to determine the optimal dose (40 J/m2) and time point (2 h) for Egr1 expression. The upper panel shows the time course and the lower panel shows the dose response of Egr1 expression upon UV-C stimulation. (b) Western analysis using anti-Egr1 to define time of maximum UV response. (c) Western analysis of M12 cells treated with UV-C or not (control) in the presence and absence of ERK1/2 inhibitor (ErkI). The results show that prior treatment with ErkI resulted in negligible induction of Egr1 upon UV stimulation. Hence, at least 90% of Egr1 induction in these cells was downstream of the ERK MAP kinase.