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Table 3 Viability loss in various mutants after methyltransferase induction

From: Cell death upon epigenetic genome methylation: a novel function of methyl-specific deoxyribonucleases

 

Viability (relative)

E. coli strain

0% arabinose

0.0002% arabinose

rec +

2.5, 2.3

1.9, 0.92

ΔrecA

1.3, 1.7

0.45, 0.31

ΔrecBC

1.3, 1.2

0.43, 0.59

lexA(Ind-)malF-

3.1, 2.5

0.21, 0.15

malF -

2.1, 2.1

0.85, 0.88

lexA(Def)sulA-

2.1, 2.0

0.96, 0.99

sulA -

2.1, 2.0

1.4, 1.2

mutS -

2.0, 1.8

1.4, 1.2

  1. Viability of several mutant E. coli strains after induction of PvuII methyltransferase was measured. The number of viable cells was monitored 30 minutes after addition of the lower concentration (0.0002%) of arabinose in two independent experiments. Each value was divided by the value at time zero.