Cell death upon epigenetic genome methylation: a novel function of methyl-specific deoxyribonucleases

Table 1 Plasmids

Plasmids	Prototype	Relevant characteristics	Drug resistance	Source, reference
pBR322	pBR322		Ap, Tc	Laboratory collection [107]
pUC19	pUC19		Ap	Laboratory collection [108]
PACYC184	pACYC184		Cm, Tc	Laboratory collection [109]
pSC101	pSC101		Tc	National Institute of Genetics [110]
pBAD18	pBR322	P_BAD	Ap	National Institute of Genetics [51]
pIK8004	pBR322	NotI linker (GCGGCCGC) in DraI site	Ap	M. Kawai (our laboratory)
PYNEC302	pUC19	pvuIIR ^- MC	Ap	Y Nakayama [19]
PYNEC313	pBR322	pvuIIRMC	Ap	Y Nakayama [19]
PYNEC404	pUC19	bamHIR ^- MC	Ap	Y Nakayama [19]
pNY43	pBR322	ecoRIIR ^- M	Ap	Y Naito [111]
pNY44	pBR322	ssoIIR ^- M	Ap	Y Naito [111]
pEF1	pBR322	P_BAD, pvuIIM	Ap	This work
pEF23	pBR322	P_BAD, pvuIIM	Ap	This work
pEF24	pSC101	P_BAD, pvuIIM	Ap	This work
pEF30	pBR322	bamHIR ^- MC	Ap	This work
pEF33	pBR322	No PvuII site	Ap, Tc	This work
pEF43	pBR322	pvuIIR^-MC, no PvuII site	Ap	This work
pKD13		OriRγ	Ap, Km	E. coli Genetic Stock Center [90]
pKD46		pSC101(Ts) ori, araC-P_BAD-redαβ	Ap	E. coli Genetic Stock Center [90]
pCP20		pSC101(Ts) ori, P_r-FLP	Ap	E. coli Genetic Stock Center [112]
pBAD30	pACYC184	P_BAD	Cm	National Institute of genetics [51]
pSI4	pUC19	sinIRM	Ap	C. Karreman [113]
pNW106RM2-3	pBR322	mspIRM	Ap	New England Biolabs [114]
pEF46		P_BAD-mcrBC	Cm	This work
pUC4K	pBR322		Ap, Km	Laboratory collection [115]
pEF60	pBR322		Km	This work
pPvuCat16	pPvu1	pPvu1 ori, pvuIIM	Cm	Robert Blumenthal [43]
pPvuCat17	pPvu1	pPvu1 ori	Cm	Robert Blumenthal [43]
pEF65	pPvu1	pPvu1 ori, pvuIIM	Km	This work
pEF67	pPvu1	pPvu1 ori	Km	This work

Ap, ampicillin-resistance; Cm, chloramphenicol-resistance; Km, kanamycin-resistance; Tc, tetracycline-resistance; Ts, temperature-sensitive.

ISSN: 1474-760X