Figure 7
McrBC-mediated cell death with DNA methyltransferases. Cells (BIK18308) harboring pEF46 (PBAD-mcrBC; see Materials and Methods) and pEF43 (M.PvuII), pSI4 (M.SinI), pNW106RM2-3 (M.MspI), or pBAD30 (vector) were streaked on LB agar plate containing 30 μg/ml chloramphenicol and 25 μg/ml ampicillin, and 0.2% glucose or 0.2% arabinose. Plates were incubated overnight at 37°C.