Changes in protein expression during honey bee larval development

Table 4 Enrichment analysis of protein classes following hierarchical clustering

Node number	Correlation	Proteins in this node	Protein class	Class total	Percent class enrichment
Solid tissue
265	0.98	5	Helicase	3	67
277	0.98	8	Hormone synthesis	4	50
376	0.94	79	Transcription	3	100
376	0.94	79	Chromatin-associated protein	3	67
376	0.94	79	tRNA synthetase	3	67
376	0.94	79	Pentose phosphate pathway	4	50
376	0.94	79	Ubiquitylation	4	50
377	0.94	5	Food	6	50
411	0.90	108	Aldo-keto reductase superfamily	3	67
419	0.89	137	Proteasome	24	67
419	0.89	137	Antioxidant	16	50
419	0.89	137	Protein receptor	4	50
421	0.89	29	ATP synthase	10	60
427	0.88	34	Small molecule receptor	4	50
434	0.86	51	Energy storage	5	80
434	0.86	51	Beta-oxidation	8	50
438	0.83	7	Cuticle	7	57
439	0.83	146	Ras superfamily	10	50
Hemolymph
128	0.97	35	Translation	7	57
150	0.96	6	Short-chain dehydrogenase family	4	50
180	0.93	21	Small molecule receptor	4	50
183	0.92	23	Food	8	63
183	0.92	23	Glycolipid metabolism	3	67
185	0.92	9	Ubiquitylation	4	50
190	0.89	63	Amino acid metabolism	8	50
197	0.86	29	Energy storage	4	100
200	0.84	81	Proteasome	10	90
200	0.84	81	Protein folding	20	60
200	0.84	81	Ribosome	31	81
203	0.82	21	Tricarboxylic acid cycle	4	75

Proteins that were manually categorized by function were subjected to average-linkage clustering (Figure 3). Separate analyses were done for solid tissue and hemolymph. Only proteins that were quantified for at least 4 out of 5 days and protein classes that had at least 5 members were considered for enrichment analysis. Classes with at least 50% enrichment in nodes with a correlation of >0.8 are considered significant and shown.

ISSN: 1474-760X