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Figure 1 | Genome Biology

Figure 1

From: Comparative sequence analyses reveal sites of ancestral chromosomal fusions in the Indian muntjac genome

Figure 1

FISH mapping of Indian muntjac BACs. Three Indian muntjac BACs (whose sequences correspond to accession numbers (a) [GenBank:AC154146], (b) [GenBank:AC152355], and (c) [GenBank:AC154920]) were mapped by FISH to metaphase spreads prepared from an Indian muntjac fibroblast cell line. Hybridization is seen at: (a) interstitial positions on chromosomes 1 (arrows), 3, and 3+X, as well as the centromere of chromosome 3 (the signals on 3 and 3+X are indicated with arrowheads); (b) an interstitial position on chromosome 1 (arrows) and at the neck of chromosome 3+X (arrowhead); and (c) multiple sites on various Indian muntjac chromosomes (arrowheads). FISH composite images generated from merging the DAPI (blue) and Spectrum Orange (red) channels (left) and inverted DAPI banding images (right) are provided in each case. Based on further studies (see text for details), the origins of the analyzed BACs were ultimately found to be on chromosome 1 in the case of (a, b), but not yet established in the case of (c); further, the analyzed clones were found to contain the indicated ancestral chromosome fusion sites (IMFS1, IMFS3, and IMFS5, respectively; Table 1).

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