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Figure 3 | Genome Biology

Figure 3

From: A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2in chromosome end protection

Figure 3

Validation of microarray data. (a) RNA from a single set of time course samples (CDC13+ (DLY3108; filled circles) and cdc13-1 (DLY3102; open circles)) was subjected to quantitative RT-PCR. Transcript levels of PNC1, UBI4, MAG1, RNR3, and YKL161C were analyzed in triplicate. Error bars represent the standard deviations of the means. Correction factors to normalize RNA concentrations were generated by calculating the geometric means of ACT1 and PAC2. A single T = 0 sample from the CDC13+ strain was assigned the value of 1 and all other values were corrected relative to this. (b) Normalized expression values from the microarray experiment of the five genes of interest quantified and plotted as in (a).

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Genome Biology

ISSN: 1474-760X

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