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Figure 1 | Genome Biology

Figure 1

From: A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2in chromosome end protection

Figure 1

Comparison of 30°C and 36°C as restrictive temperatures. (a) Two independent cultures of a cdc13-1 strain (DLY1622) grown at 23°C, were sampled. One culture was transferred to 30°C (filled triangles) and the other to 36°C (open triangles). Fractions of each culture arrested at medial nuclear division (MND) are shown. (b) cdc13-1 (DLY1622; open circles) and CDC13+ (DLY1584; filled circles) strains, grown at 23°C, were transferred to 30°C and samples taken as indicated. RNA was prepared and HSP12 transcripts were quantified using one-step quantitative RT-PCR. Plotted values represent the means of three independent measurements of each sample and error bars represent the standard deviations of the means. Correction factors to normalize HSP12 RNA concentrations of each sample were generated by calculating the geometric means of three loading controls, ACT1, PAC2 and BUD6. A single T = 0 sample from the CDC13+ strain was assigned the value of 1 and all other values were corrected relative to this. (c) This experiment was carried out as described in (c), except cdc13-1 and CDC13+ strains were transferred to the restrictive temperature of 36°C.

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Genome Biology

ISSN: 1474-760X

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