Figure 5

Nodal network anchors orchestrate coordinated recruitment of specialized ontological classes to secure a developmental theme. (a) Left: a five group K-means algorithm, 4 × 6 SOM, and QT filter were each used to independently dissect the transcriptome of embryonic stem cell (ES) derived cardiogenesis. Cardiopoietic (CP) network nodes previously identified were then used to guide cluster extraction. Bmp4 and Pitx2, members of the TGFβ pathway, are shown as examples. *Venn diagram of K-means, SOM and QT generated lists resolved clustered genes with correlated expression profiles (R = 0.95). For each set, gene (node) identity used to extract associated profiles is indicated, along with number of probes per cluster given in parentheses. Right: genes within the Bmp4 cluster. CM, cardiomyocyte. (b) Nodes belonging to the integrin cascade select discrete clusters with distinct trends. (c, d) Gene groups associated with Tgfbr2 and Vcl nodes that represent WNT/β-catenin and VEGF signaling, respectively. (e) Gene clusters organized functional neighborhoods with ontological priorities, with level of significance indicated on right.