Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome

Table 1 Summary of RACE sequencing using polyA+ and total RNA from human cell lines and tissue

Experiment	Number of exon primers	Number of novel TAR primers	Number of nonTx primers	Number of sequence reads	Number of detected transcripts on the genome
1: NB4 total RNA	34	39	0	291	154
2: Hela polyA RNA	0	59	0	273	112
3: placenta total RNA	32	20	44	195	85
4: placenta polyA RNA	0	96	96	591	147

nonTx, region not previously shown to be transcribed; RACE, rapid amplification of cDNA ends; TAR, transcriptionally active region.

ISSN: 1474-760X