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Figure 2 | Genome Biology

Figure 2

From: Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome

Figure 2

Distribution of RACE product sequences in the DRG1 and FBX07 regions. (a) DRG1 Region and (b) FBX07 region. Products from the sense strand (+) are shown in the top half of the panel. Products from the antisense strand are in the bottom half of the panel. Blue products are detected sequences from 5'-rapid amplification of cDNA ends (RACE); red products are detected sequences from 3'-RACE; black indicates refSeq; black asterisks indicate consensus splice sites (GT-AG, GC-AG, or AT-AC); and green asterisks indicate novel isoforms with more than 50% consensus splice sites. Note that the antisense products that lack consensus splice sites are indicated in lighter colors.(c) cDNA and RNA hybridization signals in DRG1 region. The blue tracks indicate the signals that were generated from hybridization of cDNA prepared from NB4 cells using reverse transcriptase to the strand-specific microarray. The red tracks indicate hybridization of RNA that has been labeled directly by chemical means, thus omitting the use of reverse transcriptase, to the strand-specific microarray. Products from the sense strand (+) are shown in the top half of the panel. Products from the antisense strand are in the bottom half of the panel.

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