Figure 5

Transcriptional, translational, and post-translational regulation of HNF1α and HNF4α during HepaRG cell differentiation. (a,c) Modulation of HNF1α (a) and HNF4α (c) in total and polysome-bound RNA populations throughout differentiation, assessed by microarray and by quantitative PCR. For microarray data, values and error bars correspond to the mean ± SEM of three independent differentiation experiments. For real-time PCR data, values and error bars correspond to the mean ± SEM of three independent measures. (b,d) Protein expression levels and phosphorylation status of HNF1α (b) and HNF4α (d) in proliferative (P) and differentiated (D) cells. The percentage of the phosphorylated forms is indicated. Results are representative of three independent differentiation processes.