Flowchart describing experimental subcellular localization data acquisition. Experimental data were generated by expressing proteins in HeLa cells and determining their subcellular localization. Proteins that localized to the cytoplasm in HeLa cells were then expressed in MCF7 cells. Proteins reported to localize to the nucleus in the LOCATE database were also included in this dataset. Ultimately, all nuclear proteins were combined, resulting in a set of 1,529 proteins.