Quantification and characterization of interstitial fibrosis in subcutaneous WAT (scWAT) of morbidly obese subjects. (a-c) Low magnification pictures of adipose tissue connective areas (stained with picrosirius, red) in a lean control and an obese patient before and three months after bariatric surgery. Pictures are representative of ten analyzed subjects. (d) Automated software quantification of picrosirius areas. Error bars indicate the upper limit of the 95% confidence interval of the mean percentage of fibrosis (e,f) Appearance of stromal connective tissue at higher magnification (e) and by electron microscopy (f) showing layer shaped fibers.