Figure 6

Chromosomal integration in mammalian culture cells. A plasmid DNA containing the transposase cDNA under the control of a ubiquitous promoter (CAGGS) and a transposon-donor plasmid DNA containing a Tol2 construct with a ubiquitous promoter (the phosphoglycerate kinase [PGK] promoter used by Kawakami and Noda [33]) and the neomycin-resistance gene are introduced into mammalian culture cells by electroporation. The Tol2 construct is excised from the plasmid and integrated into the genome. Chromosomal integration of the Tol2 construct is enhanced, depending on transposase activity [33].