Figure 3
From: Germline mutagenesis mediated by Sleeping Beautytransposon system in mice
Two possible schemes to account for the absence of GFP signal in double transgenic mice. In scheme I the integration step is extremely inefficient compared with the excision step. In scheme II both integration and excision steps are efficient, but the green fluorescent protein (GFP) signal is below the detection level, or suppression of the GFP gene is maintained at the new integration site.