SB vector microinjection. (a) Sleeping Beauty (SB) transgenesis vector. The insertion cassette containing an expression cassette that includes the gene of interest (GOI) and a reporter cassette is flanked by two SB inverted/direct repeats (IRDRs). (b) SB gene trap vector containing a splice acceptor site preceding a reporter gene. (c) SB enhancer trap vector. A minimal promoter drives expression of a reporter gene. SB vectors are co-injected with SB mRNA into one-cell-stage embryos. MP, minimal promoter; P, promoter; pA, polyadenylation signal; REP, reporter gene; SA, splice acceptor site.