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Figure 4 | Genome Biology

Figure 4

From: Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives

Figure 4

A transposition mediated system for creating chromosomal rearrangements in Arabidopsis. (a) Structure of the donor locus. (b) Creation and detection of chromosomal deletions. (c) Creation and detection of chromosomal inversions (for details, see text). Ac-ITR, Ac element inverted terminal repeat; CsR and CsS, chlorsulfuron resistance gene (active and silenced, respectively); GnR and GnS, gentamicin resistance gene (active and silenced, respectively); loxP, recognition site of the Cre recombinase; P, promoter; pA, poly(A); PptR and PptS, phosphonothricin resistance gene (active and silenced, respectively).

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