Figure 3

FLP/FRT site-specific recombination-mediated chromosomal rearrangements in Drosophila. The FRT sites are introduced into the genome via P element transposition. (a) Creation of deletions with well defined end-points. Two fly lines bearing FRT sites within P elements localized at different positions on the same chromosome are crossed. Subsequently, FLP creates the desired deletion. (b) Generation of mitotic clones in Drosophila. In this arrangement, the FRT sites are in identical positions on the homologous chromosomes of which one parental carries the mutation of interest. FLP, flip recombinase; FRT, FLP recombinase target.