Figure 4

Expression domains of common response genes and symptoms associated with infection. pF44A2.3::GFP expression in the (a) ventral nerve-cord, (b) hypodermis, (c-d) P12.pa pre-anal cells, (e-f) glial amphid socket cells, (g-h) excretory duct cell and (i) vulE or uv1 cells. Red fluorescence comes from the pcol-12::dsRED co-injection marker. In areas where both GFP and dsRED are expressed, yellow is observed. (j,k) Vacuoles (arrows) can be observed within intestinal cells of P. luminescens-infected adults (j), in which there is detectable expression of asp-4::GFP (k). Similar results were obtained with infected adults expressing asp-3::GFP. In contrast, no GFP expression or vacuolization was seen in the intestines of non-infected worms.