Figure 3

Coefficients of determination (R²) for individual hybridizations. (a) Scatter plot of a representative hybridization of a single myo-3::green fluorescent protein (GFP) replicate (Rep1) to the average intensities for all three myo-3::GFP (M0) hybridizations. (b) Results of a single myo-3::GFP hybridization (red) compared with average reference intensities (green) to identify transcripts exhibiting differential expression. Known muscle genes unc-54, myo-3, and unc-15 (top circles) are enriched in myo-3::GFP muscle cells, whereas the neuronal transcript encoding SNAP-25 is depleted (bottom circle). (c) R² values for pair-wise comparisons of myo-3::GFP M0 datasets (average = 0.92). (d) R² values for pairwise myo-3::GFP M24 datasets (average = 0.87).