Figure 2

Isolation of myo-3::GFP muscle cells by FACS. (a) myo-3::green fluorescent protein (GFP) expression in the body wall muscle cells of a newly hatched L1 larva. (b) Combined DIC and fluorescence image of a 24-hour culture of myo-3::GFP muscle cells. Panels c to e show fluorescence-activated cell sorting (FACS) profiles. (c) Fluorescence intensity scatter plot of wild-type (non-GFP) cells. Boxed areas exclude autofluorescent cells (gray). (d) myo-3::GFP cells (green) are gated to exclude propidium iodide (PI) stained cells (red). (e) Light scattering gate for GFP-positive cells (circle) to exclude cell clumps and debris. (f) myo-3::GFP muscle cells after enrichment by FACS. Scale bars: 5 μm.