Figure 6

Analysis of the vascular and haematopoietic cell gene markers in the BMP2⁺ cell population. (a) RT-PCR analysis of the BMP2⁺ cells. (b) Relative expression levels of the genes presented in (a) as obtained by Affymetrix analysis. The expression levels of each gene were normalized with its maximum level set as 100%. Each result is an average of three independent experiments (Additional data file 13). (c) Immunuostainings with anti-pan cytokeratin over the period of time to show the presence of epithelial like cells, from left to right: one day after plating the BMP2⁺ cells (7 + 1 days in total); 8 days after plating with puromycin (7 + 8 days in total); 18 days after plating with puromycin (7 + 18 days in total); and 11 days after plating without puromycin (7 + 11 days in total).