Figure 4

Synthesis of phylogenetic and karyotypic data. Tree topology from Figure 2c overlaid with chromosome rearrangements from Figure 3 and patterns of sequence expansion and contraction as seen from FISH with sat1, B29 and sat23 (right) probes to full karyotypes of representative Macropus species. Predominant FISH images are shown on the right, and chromosomes are ordered sequentially (see Additional data files 3-5 for the complete set). Ten rearrangements are indicated on branches as fusions (^) or translocations (*). The positions of the ancestral karyotypes α, β, and γ relevant to this tree are marked. Predominant centromere satellite accretion (+, gain) and diminution (-, loss) is marked by grey and white boxes. The incongruity between the concatenated gene phylogeny and the MGR tree is that the γ ancestor karyotype must have been formed twice and independently in the W. bicolor and M. giganteus lineages as shown here. Numbered arrows identify chromosome rearrangements coincident with shifts in satellite content. The major satellite constituent for each macropodine group is indicated to the far right.