Figure 1

Changes in intracellular nucleotide concentrations in induced (grey bars) and non-induced (black bars) cultures of (a) M653 [ΔrelA tipAp::relA(1.46 kb)] and (b) M653 [ΔrelA tipAp::]. Cultures were grown to an OD₄₅₀ of approximately 0.5 before treatment with 25 μg ml^-1 thiostrepton (induced) or DMSO (non-induced), and intracellular levels of nucleotides measured by HPLC analysis of extracts of cells harvested at 0, 30, 60 and 90 minutes. Values shown are in pmol mg^-1 dry cell weight and are the average of biological triplicate experiments for (a) and duplicates for (b), with standard deviations marked with error bars.