Figure 2

Data flow for the construction of the sheep virtual genome. Except where indicated in the colored boxes, all analyses were on the framework of the human genome. Sheep BAC end sequences are represented by short arrows, with the arrowheads located at the 3' end of the BAC end sequence. Paired ends are linked by dotted lines. Red arrows indicate where the sheep sequence was used in the analysis; the black (human), blue (dog), and green (cow) arrows indicate where the coordinates based on the respective genomes, or their conversion to the equivalent human or virtual sheep genome coordinates, were used in the analysis. The Xs represent BAC end sequences from the unpaired-in-cow group that have not been positioned anywhere, but are predicted to lie in the indicated position and orientation on the relevant genome. Gray arrows indicate the location of BAC end sequences within a BAC-CGC and are linked in pairs by dotted lines. The segments of framework genome, and the BAC-CGCs are colored as above and in addition pink for the virtual sheep genome. The arrowheads on the BAC-CGCs represent the orientation of the BAC-CGC relative to the human genome. Dashed lines with double headed arrows between segments of the human genome indicate path to create the virtual sheep genome. BAC, bacterial artificial chromosome; CGC, comparative genome contig.