Skip to main content

Table 1 PCR microarray and qRT-PCR values across the CENP-A major and minor domains

From: Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres

PCR fragment namea

Distance from fragment 1b

Size (bp)

qRT-PCR value (Figure 3)

CENP-A ChIP cut-off ≤ 0.68, positive ≥ 1.02c

CENP-C ChIP cut-off ≤ 0.31, positive ≥ 0.65c

CENP-H ChIP cut-off ≤ 0.56, positive ≥ 0.63c

UCSC Human Genome Browser (hg17) genome coordinates

1

0

569

 

0.72(-/+)

1.23(+)

0.59(-/+)

101,899,991-101,900,560

(1)

316

247

1.01(+)

   

101,900,060-101,900,307

2

2,064

566

 

0.51(-)

1.03(+)

1.03(+)

101,901,489-101,902,055

(2)

1,965

150

1.15(+)

   

101,901,806-101,901,956

C

6,166

178

1.78(+)

   

101,905,979-101,906,157

3

7,462

796

 

0.60(-)

2.28(+)

1.17(+)

101,906,657-101,907,453

(3)

7,184

213

2.22(+)

   

101,906,962-101,907,175

4

8,225

706

 

1.34(+)

1.70(+)

1.62(+)

101,907,510-101,908,216

D

12,840

223

1.80(+)

   

101,912,608-101,912,831

10

15,300

423

 

0.31(-)

0.40(-)

0.15(-)

101,914,868-101,915,291

11

16,222

649

 

0.71(-/+)

0.67(+)

0.72(+)

101,915,564-101,916,213

(11)

15,830

247

2.76(+)

   

101,915,574-101,915,821

26

53,983

861

 

1.17(+)

0.83(+)

0.64(+)

101,953,974-101,954,835

27

55,119

610

 

1.21(+)

1.74(+)

1.53(+)

101,954,501-101,955,110

(27)

54,554

190

3.44(+)

   

101,954,355-101,954,545

35

80,686

649

 

-1.21(-)

0.07(-)

-0.25(-)

101,980,028-101,980,677

(35-1)

80,226

172

0.86(-)

   

101,980,045-101,980,217

(35-2)

80,432

203

0.73(-)

   

101,980,220-101,980,423

36

81,822

450

 

1.72(+)

1.88(+)

1.82(+)

101,981,363-101,981,813

37

82,493

350

 

1.17(+)

1.56(+)

1.56(+)

101,982,134-101,982,484

38

85,610

786

 

-0.03(-)

0.41(-/+)

0.50(-)

101,984,815-101,985,601

(38)

85,501

143

0.48(-)

   

101,985,349-101,985,492

39

87,099

924

 

1.62(+)

0.50(-/+)

0.93(+)

101,986,166-101,987,090

40

87,859

779

 

1.49(+)

0.76(+)

1.19(+)

101,987,071-101,987,850

90

246,368

430

 

-0.06(-)

0.29(-)

0.04(-)

102,145,930-102,146,359

(90)

246,170

231

1.12(+)

   

102,145,930-102,146,161

91

250,586

597

 

1.21(+)

0.80(+)

0.82(+)

102,149,980-102,150,577

(91)

250,432

187

1.43(+)

   

102,150,236-102,150,423

92

251,863

534

 

1.33(+)

1.07(+)

1.14(+)

102,151,320-102,151,854

(92)

251,830

236

1.34(+)

   

102,151,585-102,151,821

S

253,879

158

2.65(+)

   

102,153,712-102,153,870

T

255,702

189

4.22(+)

   

102,155,504-102,155,693

93

258,459

481

 

1.14(+)

0.65(+)

0.37(-)

102,157,969-102,158,450

(93)

258,331

245

2.30(+)

   

102,158,077-102,158,322

U

259,157

224

0.99(+)

   

102,158,924-102,159,148

V

265,411

169

0.26(-)

   

102,165,233-102,165,402

  1. aNumbers indicate polymerase chain reaction (PCR) microarray fragments as in Figure 2; numbers in parentheses indicate quantitative real-time (qRT)-PCR fragments wholly contained within the PCR microarray fragment with same number (Figure 3); letters indicate qRT-PCR fragments only (Figure 3). Different regions within the major domain and the minor domain are indicated by alternating bold and regular rows. bDistances are taken from the first coordinate in fragment 1 to the last coordinate of the next fragment. cValue given in Figure 2 for PCR microarray chromatin immunoprecipitation (ChIP): (+), ≥ threshold for positive values; (±), weakly positive, over cut-off but below the threshold for positive values; (-), below the cut-off value. CENP, centromere protein.
Back to article page

Genome Biology

ISSN: 1474-760X

Contact us