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Table 2 Genomic PCR primers

From: Meta-analysis of primary target genes of peroxisome proliferator-activated receptors

Gene (region)

Location

Primer sequences (5'-3')

ACOX1 (distal)

-4919 to -4643

TGAGCTCTTGATCTCCTCCTCAGAGTCATAG

GAGTCTAGACTGGCAATCTTAGCAGAGTTC

ACOX1 (published)

-1646 to -1374

TGAGCTCTTGAACTAGAAGGTCAGCTGTC

GGGTCTAGACTAGCCTGTCTGTAGTCTGTG

ACOX1 (intron 1)

+599 to +716

TGAGCTCTTGTGATTCAGGGAGGGTGGAAC

GGGTCTAGACTGGCTGCGAGTGAGGAAG

ACOX1 (intron 2)

+2822 to +3154

TGAGCTCTTGAGATAGAGTAACTCCTCCTAG

GAGTCTAGAGAAGTGTGTCAAAGGGTGTG

ANGPTL4 (distal)

-6765 to -6535

TGAGCTCTTGAACTAGAAGGTCAGCTGTC

GAGTCTAGAATACACTCATGCAGGGTGAGG

ANGPTL4 (cluster)

+2829 to +3610

TGAGCTCTTCTCCGTTCATCTCGAACCAC

GAGTCTAGACATCTCAGAGGCTCTGCCTG

APOC3 (distal 1)

-6429 to -6143

TGAGCTCTTGCTCAGGCGATAGTTAGAAG

GAGTCTAGACTGGATGGTCCCACTCCAGTG

APOC3 (distal 2)

-4249 to -3886

TGAGCTCTTGACTATGAGGTGACATCCAGG

GAGTCTAGAGGACACACAGGCAGTACGTG

APOC3 (proximal)

-870 to -568

TGAGCTCTTGGCAGTGAGGGCTGCTCTTC

GGGTCTAGACATCTCTGGGTTTCAATCCAG

APOC3 (published)

-262 to -3

ATTTCTAGACAGTCAGCTAGGAAGGAATGAG

GGGTCTAGACTAGGGATGAACTGAGCAGAC

APOC3 (intron)

+2424 to +2722

TGAGCTCTTGATCACACAACTAATCAATCCTC

GAGTCTAGACTCAACTTCACTGGACGACAG

APOA1 (proximal)

+7701 to +8022 (relative to APOC3 TSS)

TGAGCTCTTCCTTCTCGCAGTCTCTAAGC

GAGTCTAGAGCCAACACAATGGACAATGG

CPTIB

-306 to -64

ATTTCTAGACAGAGTCTCGTGAGGATGGTG

GGGTCTAGAGTTAGCGTTCATGCTGCCAG

PPARa (distal)

-1376 to -1156

TGAGCTCTTCTGGCTAACATGTGCAAGAG

GGGTCTAGACACTGTGCTATTTGTGGCAG

PPARa(proximal)

-938 to -634

TGAGCTCTTCTCCTTGCTCTGGCAGAGTC

GGGTCTAGACTCAGAAGTGCGTAGGGTG

RVRa(distal)

-7279 to -7040

TGAGCTCTTGACCTTCCCAAGCCAAGAAC

GAGTCTAGACACTAACCTCACAGACCACTG

RVRa(proximal)

-510 to -70

TGAGCTCTTCTGGAGGTGTTCTCCCTAAG

GTGTCTAGACTGCGCAACGACAAGACTG

RVRa(TSS)

-510 to +119 (subcloned -266 to +119)

TGAGCTCTTCTGGAGGTGTTCTCCCTAAG

GTGTCTAGATTTCACTCTGCCAATCTCAGC

SULT2A1

-6104 to -5797

ATTTCTAGACTTGAATGGAAATGCCTGCTC

GGGTCTAGAGACTGGGAAGTGGGAGGAGT

UCP3 (distal)

-9680 to -9349

TGAGCTCTTCTCTAGTCTAAGTGCCTTGTC

GAGTCTAGAGTAACAGTGAGCCTCTGGTCTG

UCP3 (proximal)

-396 to -89

TGAGCTCTTGTACCTATCTCATAGGATTGTG

GTGTCTAGAGTTGACAGCCTGATCACTTGAC

UCP3 (cluster)

+2036 to +2303

TGAGCTCTTCAGGACTATGGTTGGACTGAAG

GGGTCTAGAGATGGGAGGAGGCAAGGAAG

UCP3 (intron)

+5971 to +6236

TGAGCTCTTCTCGTGCTGAGCACTTTACAC

GAGTCTAGACACTTGTTGGGTCCATTCTAAC

LASS1 (region 1)

-5297 to -4917

TGAGCTCTTCTGATGTGCAATCTCAGACAG

GAGTCTAGACTCAGTCTCCACCATGAAGG

LASS1 (region 2)

-2819 to -2499

TGAGCTCTTCCTCCCAGATGTCACCATTG

GAGTCTAGACCTCTTTTGCCACTTCCCTC

LASS1 (region 3)

-1389 to -978

TGAGCTCTTGTGGAACAGGAGCCATAGAG

GGGTCTAGACATCGAGGAAGACACTGGTC

  1. Sequence and location of the primer pairs used for real-time PCR quantification of genomic regions containing putative REs within the nine PPAR target genes. The positions indicated are in relation to the respective annotated gene TSS. The same primers were used for subcloning; the gene-specific sequences are indicated in bold.