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Figure 3 | Genome Biology

Figure 3

From: Meta-analysis of primary target genes of peroxisome proliferator-activated receptors

Figure 3

In silico analysis of selected primary PPAR target genes. Overview of the genomic organization of eight human PPAR target genes; 10 kB upstream and downstream of the TSSs are shown (horizontal black line). Putative REs (red boxes, no conservation; orange boxes, within conserved area; yellow boxes, conserved) were identified using the classifier by in silico screening of the genomic sequences and are classified according to their degree of conservation compared to the orthologous mouse gene. Already published PPREs are indicated by an asterisk. For each predicted RE the calculated binding strengths of PPARα (green), PPARγ (dark blue) and PPARβ/δ (light blue) in reference to a consensus DR1-type PPRE are represented by column height. All putative PPRE sequences are available on request. For the UCP3 gene REs, the average in vitro DNA binding strength of PPAR-RXR heterodimers was also determined by gelshift experiments and is shown in the same color code scheme. Horizontal red bars indicate the genomic regions that were subcloned for reporter gene assays (Figure 4) and were analyzed by ChIP assays (Figure 5).

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