Figure 6From: High-throughput analysis of spatio-temporal dynamics in DictyosteliumThe screen uncovers mutants with aberrant slug motion. The multicellular slug phenotype is often difficult to see in cells feeding on bacterial lawns (left-hand panel) because development is asynchronous and the slug stage is transient. The middle panels are snapshots from our automated imaging system taken at around 24 h. Slug trajectories over a 28.5-h period were obtained by first binary thresholding the movies and then tracking the center of mass by multiple particle tracking using ImageJ (right-hand panel). Data shown are from mutant clones recreated by homologous recombination.Back to article page