Figure 5

Module I contains a 5' to 3' organized sequence of cis-regulatory elements that control the posterior to anterior expression of olSix3.2 in brain. (a) The drawings illustrate the design of the cXXIII to cXXIX constructs use to determine the arrangement of the cis-regulatory elements within module I, using five progressive deletions of about 50 base pairs, indicated by a gradient of blue colors. (b) Nucleotide sequence of module I, in which the precise position of the deletions is indicated with the same gradient of blue colors. (c to g) Epi-fluorescence dorsal views of cXXIV to cXXIX transgenic embryos that show the loss of thalamic (panel d), hypothalamic (panels e and g), and telencephalic (panels f and g) reporter expression. cXXVII transgenic embryos exhibited no enhanced green fluorescent protein (EGFP) expression. Hy, hypothalamus; Te, telencephalon; Th, thalamus.