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Figure 3 | Genome Biology

Figure 3

From: Genetic background influences murine prostate gene expression: implications for cancer phenotypes

Figure 3

Analysis of strain-dependent differences in prostate gene expression by qRT-PCR. RNAs from preparations used in the (a) microarray analysis or (b) microdissected epithelium were reverse transcribed and amplified using qRT-PCR with primers specific for seminal vesicle secretion 2 (Svs2), matrix metallopeptidase 7 (Mmp7), prostate stem cell antigen (Psca) and spermine binding protein (Sbp). Ribosomal protein S16 expression levels were used to normalize qRT-PCR data. Normalized results are expressed relative to the lowest expressing value. Error bars indicate the standard deviation of four biological independent replicates. qRT-PCR for microdissected epithelium is represented by one sample per strain for each gene. White bars denote measurements from the microarray analysis. Black bars denote measurements generated by qRT-PCR from whole prostate. Diagonal lines denote measurements generated by qRT-PCR from microdissected prostate epithelium.

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