Figure 2

Prostate gene expression differences among strains. (a) Scatter plot of variance in gene expression levels between strains and within strains. (b) Average-linkage hierarchical clustering for the 932 differentially expressed genes among the five mouse strains (FDR <10%). Heat map colors reflect fold ratio values between sample and reference pool and mean-centered across samples. Columns represent biological replicates for each strain. Rows represent individual genes. Values shown in red are relatively larger than the overall mean; values shown in green are relatively smaller than the overall mean (see scale). Genes whose expression changes were confirmed by qRT-PCR, western blot or immunohistochemistry are listed. (c) Separation of the five strains in three-dimensional principal component space by applying PCA to the 932 genes with strain variance.