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Table 4 Summary of LongSAGE tag cluster data according to breed type

From: LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs

Tongcheng

   

Cluster*

Tags per cluster†

Previously characterized genes‡

GO categories enriched in the cluster§

1

191

APOBEC2, BIN1, BTBD1, CACNB1, MYBPC1, MYL1, MYOZ1, RRAS, RTN4, SH3BGR, SLN, TCAP, TMOD1, TNNC2, TNNT3, TPM1

Muscle development (0.0007806)

2

245

ACTN2, AMPD1, ATP2A1, CKM, DES, ENO3, MYH2, RYR1, TMOD4, TPM2, GAPDH, IGF2

Carbohydrate metabolism (0.001492) Mitochondrion (0.016297)

3

204

LASS2

Signal transduction (0.008368)

4

160

SPARC, HSPB2, ATF4, MYL2

Tricarboxylic acid cycle (0.021523489)

5

152

MYH3, S100A11

Ribosomal (1.25E-06)

6

77

  

7

218

PSMD2, RTN2, SERF1A, THBS4, TIMM13, TNNC1, TNNI1, TNNT1, FAU, HSPA8, FHL1C, HDAC5, MYOT

Obsolete molecular function (0.048234)

8

153

DGKZ, GNB2L1

Binding (0.023903)

Total

1,400

  

Landrace

   

1

158

APOBEC2, CFL2, HSPB2, ITGB1, MYH3, PGM5, RTN4, TNNT1, TPM2, FHL1C

Muscle development (0.003181)

2

94

SPARC, TIMM13

Mitochondrion (0.002539)

3

126

AMPD1, CKM, DES, MYBPC1, MYL1, MYOZ1, PFDN5, RYR1, SLN, TNNT3, MYL2

Muscle contraction (0.035242)

4

160

LGALS1, SERF1A1, FAU, HSPA8

Ribosomal (4.83E-07)

5

84

 

Cytoskeleton organization and biogenesis (0.012664)

6

301

GSN, TPM3, TPM4, TRIO, S100A11, NACA, SUMO2

Cell cycle (0.002587)

7

95

CACNB1, MYLPF, TMOD1, TNNI1, IGF2

Development (0.014224)

8

183

ACTN2, ATP2A1, BIN1, CCNG1, ENO3, MYH2, RTN2, SH3BGR, TCAP, TPM1, GAPDH, SDHD, HDAC5

Protein complex assembly (0.035886)

Total

1,201

  
  1. *The expression patterns in each cluster for Tongcheng and Landrace pigs are shown as (A) and (B) in Additional data file 6, respectively. †Tags in cluster value corresponds to the number of LongSAGE tags in each cluster. ‡Selected genes previously detected during myogenesis are indicated (references for these are given in Additional data file 7). §All significant (p < 0.05) GO categories over-represented in individual clusters were determined by EASE scores [68]; raw EASE scores (Jackknife one-sided Fisher exact p values) for the categories in question are given in parentheses.