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Table 1 Summary of data obtained from the LongSAGE libraries

From: LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs

 

LongSAGE library

 

T33

T65

T90

L33

L65

L90

Total tags1

50,450

53,927

53,761

53,104

54,483

51,188

Unique tags2

25,738

24,655

22,035

24,408

24,829

22,464

Unique tags/total tags (%)3

51.0

45.7

41.0

46.0

45.6

43.9

Tags/clone

23.4

26.5

31.7

25.97

24.8

25.9

Average GC content4

45.16

50.02

44.56

47.24

47.15

46.20

Remaining total tags5

32,722 (64.8)

37,058 (68.6)

40,074 (74.5)

37,135 (69.8)

37,948 (69.6)

36,596 (71.4)

Remaining unique tags6

8,081

7,786

8,348

8,439

8,294

7,872

Unmatched tags7

2,458 (30.4%)

2,377 (30.5%)

2,621 (31.4%)

2,362 (28.0%)

2,740 (33.0%)

2,513 (31.9%)

Matched tags8

5,623 (69.6%)

5,409 (69.5%)

5,727 (68.6%)

6,077 (72.0%)

5,554 (67%)

5,359 (68.1%)

Single match9

5,437 (96.7%)

5,249 (96.9%)

5,550 (97%)

5,887 (97%)

5,358 (96.8%)

5,163 (96.7%)

Multiple matches10

188 (3.3%)

169 (3.1%)

174 (3.0%)

185 (3.0%)

175 (3.2%)

176 (3.3%)

  1. 1Total tags were obtained in each library. LongSAGE tags containing wildcard characters not in {A, C, G, T} were discarded. 2Unique tags were obtained in each library. 3The differences in accrual rates (the ratio of unique tags to total tags) indicate that the number of genes expressed regularly changed during myogenesis. 4For an explanation of this value, in the context of the quality of a SAGE library, see Margulies et al. [31]. 5The total tags remaining in each library after eliminating the unique tags with a frequency <2 in all six libraries. The percentage of non-singleton tags is shown in parentheses. 6The unique tags remaining in each library after eliminating the unique tags with a frequency <2 in all six libraries. 7Unique tags unmatched with any known sequence. The values in parentheses indicate the percentage of unique tags in the total. 8Unique tags that correspond to UniGene entries. The values in parentheses indicate the percentage of unique tags in the total. 9Unique tags matched with a single UniGene sequence. 10Unique tags matched with more than one UniGene sequence. T, Tongcheng; L, Landrace; 33, 65 and 90 refer to days post coitus.