A semi-quantitative GeLC-MS analysis of temporal proteome expression in the emerging nosocomial pathogen Ochrobactrum anthropi

Table 4 Proteins identified within the soluble sub-proteome of O. anthropi containing predicted export signal peptides

Accesion no. (NCBI)	Function	Signal peptide
17982947	Methionyl-tRNA synthetase	MSPLTNFFS RAYHA
17983887	Dihydroxy-acid dehydratase	MKMPPYRSRTTTHGRNMAGA
17982453*	Hypothetical protein (immunoreactive 28 kDa omp)	MNTRASNFLAASFSTIMLVGAFSLPAFA
17983949	3-Deoxy-manno-oculosonate cytidylyltransferase	MVLLPPRKTARVGTRRKPVFLSQTCANG
17984491*	Lipoprotein (ABC transporter substrate binding protein)	MSSVLSRYALTRRAGLK ALLFTAAALTVGFASAPSHA
27353255	Transriptional regulatory protein	MRAFTRFSYSHS
17982679	Protein translation elongation factor Tu	MCWRLSGSRTKRTTAMA
17982015*	Malate dehydrogenase	MRKETIMARNK IALIGSGMIGG TLA
17982340*	Periplasmic dipeptide transport protein precursor	MGCARQAFPWRRTIMKFYQK LLAATALVALMSGA ASA
17982205	Transketolase	MLCVPLPSGASSR KAA
17982141	ATP synthase F1, beta chain	MAKAATPKTTAAAEA
17982826	DNA-binding protein HU alpha	MPMNKNELVA
17982154*	Leucine, isoleucine, valine, threonine and alanine binding protein	MGVPTMRKTLFSGVALAAVIAFGGSAWA
17983192*	General L-amino acid-binding periplasmic protein AAPJ precursor	MKKTLMTGVLGAAALFGIASGASA
17982682	50S ribosomal protein L11	MAKKVAGQLKLQVPAGA
17983290	Dihydrodipicolinate synthase	MLGVPSICFRSSRMLKGSITA
86283673*	Dipeptide ABC transporter, substrate binding	MMITRLSRKFR LLSAGAALSLLMMAAPSAFA

Putative signal peptides were predicted as described by Tjalsma et al. [47] and Pugsley [48]. *Proteins likely to be secreted via the Sec pathway. Signal peptide: the hydrophobic H-domain is in italics; positively charged amino acids are underlined; the signal peptide cleavage sites comprise the last three amino acid residues and are in bold.

ISSN: 1474-760X