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Table 2 Proteins identified in both growth phases with their bioinformatic analysis and emPAI calculation

From: A semi-quantitative GeLC-MS analysis of temporal proteome expression in the emerging nosocomial pathogen Ochrobactrum anthropi

Accession no. (NCBI)

Protein

Mowse

PSortB

SignalP

SecP

emPAI

Protein (M%)

Fold change

Species

  

0.3

1.2

L

SP

SP

 

0.3

1.2

0.3

1.2

  

17985267

60 kDa chaperonin GroEl

1334

1734

C

No

No

No

0.778

0.884

3.068

2.985

1.0

Bm

17982679

Protein translation elongation factor Tu

828

1133

C

No

AMA-KS17,18

No

0.897

1.153

3.537

3.893

0.9

Bm

17982693

Protein translation elongation factor G

547

884

C

No

No

No

0.459

0.503

1.810

1.698

1.1

Bm

17982686

DNA directed RNA polymerase beta chain

601

686

C

No

No

No

0.211

0.183

0.832

0.618

1.3

Bm

17982688

DNA directed RNA polymerase beta' chain

461

675

C

No

No

No

0.132

0.172

0.520

0.581

0.9

Bm

17984056

DNAK protein (HSP 70)

404

613

C

No

No

Y 0.69

0.225

0.288

0.887

0.972

0.9

Bm

17982961

30S ribosomal protein S1

541

611

U

No

No

Y 0.85

4.623

3.645

18.228

12.308

1.5

Bm

17983895

Aconitate hydratase

288

563

C

No

No

No

0.18

0.297

0.710

1.033

0.7

Bm

17981970

Electron transfer flavoprotein beta subunit

396

342

U

No

No

Y 0.63

0.469

0.469

1.849

1.584

1.2

Bm

17982110

Membrane-bound lytic murien transglycosylase B

238

103

CM

No

No

No

0.469

0.202

1.849

0.682

2.7

Bm

17984018

N utilization protein NusA

75

135

C

No

No

No

0.096

0.167

0.379

0.564

0.7

Bm

17982394

Ribose-phosphate pyrophosphokinase

95

192

U

No

No

No

0.146

0.250

0.576

0.844

0.7

Bm

17982015

Malate dehydrogenase

174

409

C

No

TLA-HL25,26

No

0.291

0.816

1.147

2.755

0.4

Bm

17982340

Periplasmic dipeptide transport protein pre

323

371

P

No

ASA-KT37,38

Y 0.93

0.39

0.51

1.538

1.722

0.9

Bm

17982978

Fumarate hydratase class I aerobic

301

309

C

No

No

No

0.406

0.291

1.601

0.983

1.6

Bm

17982732

Isocitrate dehydrogenase (NADP)

275

396

U

No

No

No

0.241

0.333

0.950

1.124

0.8

Bm

17982121

Phosphoribosylaminoimidazolecarboxamide formyltransferase

261

365

C

No

No

No

0.216

0.315

0.852

1.064

0.8

Bm

17983182

Aspartyl-tRNA synthetase

262

334

C

No

No

No

0.156

0.197

0.615

0.665

0.9

Bm

17982205

Transketolase

252

213

C

No

KAA-DG16,17

No

0.222

0.143

0.875

0.483

1.8

Bm

17982204

Glyceraldehyde 3-phosphate dehydrogenase

230

288

C

No

No

No

0.291

0.377

1.147

1.273

0.9

Bm

17983520

Enoyl-(acyl carrier protein) reductase (NADH)

232

216

C

No

No

No

0.648

0.493

2.555

1.665

1.5

Bm

17984008

Enoyl-(acyl carrier protein) reductase (NADH)

202

197

C

No

No

No

0.422

0.556

1.664

1.877

0.9

Bm

17982437

Carbamoyl-phosphate synthase large chain

125

286

U

Yes

No

No

0.038

0.161

0.150

0.544

0.3

Bm

17983107

30S ribosomal protein S4

206

62

U

No

No

Y 0.54

0.358

0.107

1.412

0.361

3.9

Bm

17982692

30S ribosomal protein S7

81

225

U

No

No

No

0.167

0.358

0.658

1.209

0.5

Bm

17985266

10 kDa chaperonin GroES

192

168

C

No

No

No

0.368

0.368

1.451

1.243

1.2

Bm

23463995

Conserved hypothetical protein

94

225

C

No

No

No

0.146

0.403

0.576

1.361

0.4

Bs

86279873

Polyribonucleotide nucleotidyltransferase protein

190

146

C

No

No

No

0.114

0.114

0.450

0.385

1.2

Re

17983037

Trigger factor, peptidylprolyl isomerase

131

224

C

No

No

No

0.089

0.119

0.351

0.408

0.9

Bm

17982138

ATP synthase F1, alpha chain

158

112

U

No

No

No

2.63

2.63

10.370

8.880

1.2

Bm

17982141

ATP synthase F1, beta chain

180

173

U

No

AEA-KP15,16

No

0.233

0.169

0.919

0.571

1.6

Bm

17984734

Glycine dehydrogenase (decarboxylating)

99

218

C

No

No

No

0.064

0.127

0.252

0.429

0.6

Bm

17982133

Transaldolase

179

218

U

No

No

No

0.374

0.374

1.475

1.263

1.2

Bm

17982713

30S ribosomal protein S5

164

133

C

No

No

No

0.138

0.089

0.544

0.301

1.8

Bm

17982705

30S ribosomal protein S17

96

205

U

No

No

Y 0.73

0.025

0.374

0.099

1.263

0.1

Bm

17983483

Malonyl coa-acyl carrier protein transacylase

160

135

C

No

No

No

0.259

0.259

1.021

0.875

1.2

Bm

17982471

ABC transporter ATP-binding protein YjjK

84

185

CM

No

No

No

0.084

0.114

0.331

0.385

0.9

Bm

17984086

Adenosylhomocysteinase

158

159

C

No

No

No

0.161

0.161

0.635

0.544

1.2

Bm

17983095

Phosphoribosylaminoimidazole-succinocarboxamide synthase

157

166

C

No

No

No

0.321

0.23

1.266

0.777

1.6

Bm

17982017

Succinyl-CoA synthetase alpha chain

155

176

C

No

No

No

0.291

0.291

1.147

0.983

1.2

Bm

17982721

DNA directed RNA polymerase alpha chain

134

65

C

No

No

No

0.211

0.138

0.832

0.466

1.8

Bm

17982016

Succinyl-CoA synthetase beta chain

76

157

C

No

No

No

0.094

0.197

0.371

0.665

0.6

Bm

17983100

Phosphoribosylformylglycinamidine synthase II

119

150

U

No

No

No

0.13

0.13

0.513

0.439

1.2

Bm

17982700

30S ribosomal protein S19

92

150

U

No

No

Y 0.9

0.291

0.469

1.147

1.584

0.7

Bm

17983486

30S ribosomal protein S18

119

63

U

No

No

Y 0.53

0.197

0.091

0.777

0.307

2.5

Bm

17982938

Glutamine synthetase I

115

63

C

No

No

Y 0.63

0.104

0.104

0.410

0.351

1.2

Bm

23463708

GMP synthase (glutamine-hydrolyzing)

113

87

C

No

No

No

0.227

0.107

0.895

0.361

2.5

Bs

17982702

30S ribosomal protein S3

57

136

C

No

No

No

0.045

0.143

0.177

0.483

0.4

Bm

17982781

Citrate synthase

112

114

C

No

No

No

0.146

0.146

0.576

0.493

1.2

Bm

17983059

Arginyl-tRNA synthetase

111

113

C

No

No

No

0.057

0.057

0.225

0.192

1.2

Bm

17982196

Hypothetical cytosolic protein

102

73

U

No

No

Y 0.69

0.14

0.069

0.552

0.233

2.4

Bm

17982768

Protein translation elongation factor Ts

99

132

C

No

No

No

0.067

0.138

0.264

0.466

0.6

Bm

17983768

Aldehyde dehydrogenase

41

111

C

No

No

No

0.089

0.138

0.351

0.466

0.8

Bm

17982113

Chorismate mutase

76

82

U

No

No

No

0.39

0.39

1.538

1.317

1.2

Bm

17983157

Integration host factor alpha subunit

57

102

U

No

No

Y 0.51

0.089

0.186

0.351

0.628

0.6

Bm

  1. Cellular localizations: C, cytoplasmic; CM, cytoplasmic membrane; E, extracellular; P, periplasmic; U, unknown. SecP, SecretomeP; SP, signal peptide. Species: Bm, Brucella melitensis; Bs, Brucella suis; Re, Rhizobium etli.
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Genome Biology

ISSN: 1474-760X

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