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Table 2 Proteins identified in both growth phases with their bioinformatic analysis and emPAI calculation

From: A semi-quantitative GeLC-MS analysis of temporal proteome expression in the emerging nosocomial pathogen Ochrobactrum anthropi

Accession no. (NCBI) Protein Mowse PSortB SignalP SecP emPAI Protein (M%) Fold change Species
   0.3 1.2 L SP SP   0.3 1.2 0.3 1.2   
17985267 60 kDa chaperonin GroEl 1334 1734 C No No No 0.778 0.884 3.068 2.985 1.0 Bm
17982679 Protein translation elongation factor Tu 828 1133 C No AMA-KS17,18 No 0.897 1.153 3.537 3.893 0.9 Bm
17982693 Protein translation elongation factor G 547 884 C No No No 0.459 0.503 1.810 1.698 1.1 Bm
17982686 DNA directed RNA polymerase beta chain 601 686 C No No No 0.211 0.183 0.832 0.618 1.3 Bm
17982688 DNA directed RNA polymerase beta' chain 461 675 C No No No 0.132 0.172 0.520 0.581 0.9 Bm
17984056 DNAK protein (HSP 70) 404 613 C No No Y 0.69 0.225 0.288 0.887 0.972 0.9 Bm
17982961 30S ribosomal protein S1 541 611 U No No Y 0.85 4.623 3.645 18.228 12.308 1.5 Bm
17983895 Aconitate hydratase 288 563 C No No No 0.18 0.297 0.710 1.033 0.7 Bm
17981970 Electron transfer flavoprotein beta subunit 396 342 U No No Y 0.63 0.469 0.469 1.849 1.584 1.2 Bm
17982110 Membrane-bound lytic murien transglycosylase B 238 103 CM No No No 0.469 0.202 1.849 0.682 2.7 Bm
17984018 N utilization protein NusA 75 135 C No No No 0.096 0.167 0.379 0.564 0.7 Bm
17982394 Ribose-phosphate pyrophosphokinase 95 192 U No No No 0.146 0.250 0.576 0.844 0.7 Bm
17982015 Malate dehydrogenase 174 409 C No TLA-HL25,26 No 0.291 0.816 1.147 2.755 0.4 Bm
17982340 Periplasmic dipeptide transport protein pre 323 371 P No ASA-KT37,38 Y 0.93 0.39 0.51 1.538 1.722 0.9 Bm
17982978 Fumarate hydratase class I aerobic 301 309 C No No No 0.406 0.291 1.601 0.983 1.6 Bm
17982732 Isocitrate dehydrogenase (NADP) 275 396 U No No No 0.241 0.333 0.950 1.124 0.8 Bm
17982121 Phosphoribosylaminoimidazolecarboxamide formyltransferase 261 365 C No No No 0.216 0.315 0.852 1.064 0.8 Bm
17983182 Aspartyl-tRNA synthetase 262 334 C No No No 0.156 0.197 0.615 0.665 0.9 Bm
17982205 Transketolase 252 213 C No KAA-DG16,17 No 0.222 0.143 0.875 0.483 1.8 Bm
17982204 Glyceraldehyde 3-phosphate dehydrogenase 230 288 C No No No 0.291 0.377 1.147 1.273 0.9 Bm
17983520 Enoyl-(acyl carrier protein) reductase (NADH) 232 216 C No No No 0.648 0.493 2.555 1.665 1.5 Bm
17984008 Enoyl-(acyl carrier protein) reductase (NADH) 202 197 C No No No 0.422 0.556 1.664 1.877 0.9 Bm
17982437 Carbamoyl-phosphate synthase large chain 125 286 U Yes No No 0.038 0.161 0.150 0.544 0.3 Bm
17983107 30S ribosomal protein S4 206 62 U No No Y 0.54 0.358 0.107 1.412 0.361 3.9 Bm
17982692 30S ribosomal protein S7 81 225 U No No No 0.167 0.358 0.658 1.209 0.5 Bm
17985266 10 kDa chaperonin GroES 192 168 C No No No 0.368 0.368 1.451 1.243 1.2 Bm
23463995 Conserved hypothetical protein 94 225 C No No No 0.146 0.403 0.576 1.361 0.4 Bs
86279873 Polyribonucleotide nucleotidyltransferase protein 190 146 C No No No 0.114 0.114 0.450 0.385 1.2 Re
17983037 Trigger factor, peptidylprolyl isomerase 131 224 C No No No 0.089 0.119 0.351 0.408 0.9 Bm
17982138 ATP synthase F1, alpha chain 158 112 U No No No 2.63 2.63 10.370 8.880 1.2 Bm
17982141 ATP synthase F1, beta chain 180 173 U No AEA-KP15,16 No 0.233 0.169 0.919 0.571 1.6 Bm
17984734 Glycine dehydrogenase (decarboxylating) 99 218 C No No No 0.064 0.127 0.252 0.429 0.6 Bm
17982133 Transaldolase 179 218 U No No No 0.374 0.374 1.475 1.263 1.2 Bm
17982713 30S ribosomal protein S5 164 133 C No No No 0.138 0.089 0.544 0.301 1.8 Bm
17982705 30S ribosomal protein S17 96 205 U No No Y 0.73 0.025 0.374 0.099 1.263 0.1 Bm
17983483 Malonyl coa-acyl carrier protein transacylase 160 135 C No No No 0.259 0.259 1.021 0.875 1.2 Bm
17982471 ABC transporter ATP-binding protein YjjK 84 185 CM No No No 0.084 0.114 0.331 0.385 0.9 Bm
17984086 Adenosylhomocysteinase 158 159 C No No No 0.161 0.161 0.635 0.544 1.2 Bm
17983095 Phosphoribosylaminoimidazole-succinocarboxamide synthase 157 166 C No No No 0.321 0.23 1.266 0.777 1.6 Bm
17982017 Succinyl-CoA synthetase alpha chain 155 176 C No No No 0.291 0.291 1.147 0.983 1.2 Bm
17982721 DNA directed RNA polymerase alpha chain 134 65 C No No No 0.211 0.138 0.832 0.466 1.8 Bm
17982016 Succinyl-CoA synthetase beta chain 76 157 C No No No 0.094 0.197 0.371 0.665 0.6 Bm
17983100 Phosphoribosylformylglycinamidine synthase II 119 150 U No No No 0.13 0.13 0.513 0.439 1.2 Bm
17982700 30S ribosomal protein S19 92 150 U No No Y 0.9 0.291 0.469 1.147 1.584 0.7 Bm
17983486 30S ribosomal protein S18 119 63 U No No Y 0.53 0.197 0.091 0.777 0.307 2.5 Bm
17982938 Glutamine synthetase I 115 63 C No No Y 0.63 0.104 0.104 0.410 0.351 1.2 Bm
23463708 GMP synthase (glutamine-hydrolyzing) 113 87 C No No No 0.227 0.107 0.895 0.361 2.5 Bs
17982702 30S ribosomal protein S3 57 136 C No No No 0.045 0.143 0.177 0.483 0.4 Bm
17982781 Citrate synthase 112 114 C No No No 0.146 0.146 0.576 0.493 1.2 Bm
17983059 Arginyl-tRNA synthetase 111 113 C No No No 0.057 0.057 0.225 0.192 1.2 Bm
17982196 Hypothetical cytosolic protein 102 73 U No No Y 0.69 0.14 0.069 0.552 0.233 2.4 Bm
17982768 Protein translation elongation factor Ts 99 132 C No No No 0.067 0.138 0.264 0.466 0.6 Bm
17983768 Aldehyde dehydrogenase 41 111 C No No No 0.089 0.138 0.351 0.466 0.8 Bm
17982113 Chorismate mutase 76 82 U No No No 0.39 0.39 1.538 1.317 1.2 Bm
17983157 Integration host factor alpha subunit 57 102 U No No Y 0.51 0.089 0.186 0.351 0.628 0.6 Bm
  1. Cellular localizations: C, cytoplasmic; CM, cytoplasmic membrane; E, extracellular; P, periplasmic; U, unknown. SecP, SecretomeP; SP, signal peptide. Species: Bm, Brucella melitensis; Bs, Brucella suis; Re, Rhizobium etli.