Table 1 Quantification of GFP expression for each reporter construct
Reporter construct | Notochord >15 cells | Notochord <15 cells | Floor plate >15 cells | Floor plate <15 cells | Ectopic >15 cells | Ectopic <15 cells | Nonexpressing | Total number |
|---|---|---|---|---|---|---|---|---|
0.8shha:gfp | 0% | 3 ± 1.6% | 0% | 2.3 ± 0.9% | 0% | 16 ± 3.5% | 84 ± 3.5% | 224 |
0.8shha:gfp:z-shha-I2 | 57 ± 2.9% | 32.9 ± 5.2% | 3.4 ± 1.2% | 86.5 ± 3% | 0% | 89.9 ± 3.8% | 10.1 ± 4.7% | 301 |
0.8shha:gfp:t-shha-I2 | 58.8 ± 3.3% | 27.1 ± 6.7% | 4 ± 0.7% | 82 ± 4.6% | 0% | 86 ± 4% | 14 ± 4.9% | 272 |
0.8shha:gfp:l-shh-I2 | 61.2 ± 8.5% | 26.4 ± 5.2% | 1.2 ± 0.3% | 86.4 ± 3.5% | 0% | 87.6 ± 3.4% | 12.4 ± 4.2% | 325 |
0.8shha:gfp:c-shh-I2 | 56.1 ± 7.2% | 28.9 ± 11.5% | 2 ± 0.1% | 83.1 ± 4.2% | 0% | 85 ± 4.3% | 15 ± 6.1% | 203 |
0.8shha:gfp:z-shhb-I2 | 30.2 ± 5.3% | 51.6 ± 6.9% | 38.1 ± 4.9% | 43.7 ± 8.9% | 2.5 ± 1% | 79.3 ± 4.6% | 18.2 ± 5.4% | 281 |
0.8shha:gfp:t-shhb-I2 | 27.9 ± 7.9% | 50.9 ± 7.9% | 37.8 ± 5.7% | 41 ± 6% | 2.1 ± 0.7% | 76.8 ± 2% | 21.2 ± 3.3% | 248 |
0.8shha:gfp:z-shhb-I2-non.cons. | 0% | 1.3 ± 1.3% | 0% | 2.1 ± 0.8% | 0% | 7.7 ± 2.4% | 92.3 ± 3.5% | 145 |
0.8shha:gfp:z-shhb-arC | 36.7 ± 5.7% | 48.9 ± 7.2% | 46 ± 5.4% | 39.6 ± 10.3% | 3.1 ± 0.3% | 82.4 ± 4.8% | 14.4 ± 6.9% | 409 |
0.8shha:gfp:z-shha-arC | 62.2 ± 5.6% | 28.6 ± 2.4% | 4.4 ± 1.1% | 86.4 ± 3.4% | 0% | 90.8 ± 3.5% | 9.2 ± 4.3% | 260 |
0.8shha:gfp:z-shha-arCΔC1 | 0% | 2.2 ± 0.6% | 0% | 1.5 ± 0.1% | 5.2 ± 0.3% | 11.9 ± 1% | 82.9 ± 0.9% | 135 |
0.8shha:gfp:z-shha-arCΔC2 | 46.2 ± 4.3% | 31.1 ± 8.8% | 5 ± 1.3% | 72.2 ± 3.3% | 0% | 77.2 ± 4.5% | 22.8 ± 5.6% | 347 |
0.8shha:gfp:z-shha-arCΔC3 | 51.2 ± 3.6% | 30.5 ± 2.6% | 47.1 ± 4.5% | 34.6 ± 5.7% | 3.7 ± 1.3% | 78 ± 1.9% | 18.3 ± 3.7% | 307 |
0.8shha:gfp:z-shha-arCΔC4 | 32.5 ± 5.1% | 48.6 ± 6.6% | 37.6 ± 3.1% | 43.5 ± 4.8% | 2.1 ± 1.3% | 79.1 ± 5% | 18.9 ± 4.7% | 359 |
0.8shha:gfp:z-shha-arC+C4m | 36.8 ± 6.2% | 41.6 ± 5.4% | 42.3 ± 7.2% | 36.1 ± 6.7% | 2.8 ± 1.6% | 75.6 ± 4.5% | 21.6 ± 5.1% | 174 |
0.8shha:gfp:z-shhb-arCΔC1 | 0% | 0% | 0% | 0% | 3.8 ± 1.6% | 10.7 ± 7.7% | 85.5 ± 11.3% | 186 |
0.8shha:gfp:z-shhb-arCΔC3 | 33.5 ± 3% | 40.5 ± 6% | 37.8 ± 3.9% | 36.2 ± 7.3% | 0% | 74 ± 3.5% | 26 ± 4.3% | 230 |
0.8shha:gfp:z-shhb-arC+C2 | 23 ± 6.2% | 44.6 ± 8.7% | 36 ± 5.2% | 31.6 ± 7.8% | 1.3 ± 1% | 66.3 ± 3.2% | 32.4 ± 3.2% | 203 |
0.8shha:gfp:z-shhb-arC+C4 | 45.7 ± 7.2% | 43.3 ± 4.7% | 8.2 ± 2.4% | 80.8 ± 3.8% | 0% | 89 ± 3.2% | 11 ± 3.9% | 288 |
2.7shhb:gfp | 72.4 ± 3.1% | 19.6 ± 3.3% | 0% | 92 ± 3.4% | 0% | 92 ± 3.4% | 8 ± 4.2% | 308 |
2.7shhb:gfp:z-shhbI1 | 68 ± 4.9% | 19.8 ± 0.8% | 0% | 87.8 ± 4.2% | 0% | 87.8 ± 4.2% | 12.2 ± 5.1% | 339 |
2.7shhb:gfp:z-shhbI2 | 61.4 ± 4.9% | 24.7 ± 2.9% | 36.4 ± 3.6% | 49.7 ± 3.1% | 2 ± 0.8% | 84.1 ± 5.6% | 13.9 ± 7.7% | 296 |