Figure 4

Genomic locations of RAD markers linked to zebrafish mutations. Wide gray vertical bars represent chromosome (Chr) regions based on the Ensembl Zv6 genome assembly (megabase positions are shown); restriction site associated DNA (RAD) marker positions (red tick marks) are indicated to the left, and microsatellite markers to the right. Narrow, vertical bars represent linkage groups (LGs) based on the MGH mapping panel [27]. Lines connect the genome assembly and genetic map positions of microsatellite markers. Crossed lines indicate inconsistencies between the genome assembly and genetic map. Microsatellite markers were used to confirm the RAD mapping results. The recombination patterns in individual embryos identified regions containing the mutations (black brackets). Microsatellite markers are shown to the right of the linkage group. The number of recombination events between the marker and mutation over the total number of meioses is shown in parentheses. (a) b1182 was isolated in the TU background and out-crossed to WIK for mapping. RAD marker positions suggest that b1182 is between 42.1 cM (z25238) and 44.2 cM (z10008) on LG 18. Recombination patterns in individual embryos confirm that b1182 is within or very near this region. (b) b1127 was isolated in the TU background and out-crossed to AB. RAD marker positions suggest that b1127 is between 59.2 cM (z7803) and 75.7 cM (z4394) on LG 20. Individual genotypes confirm b1127 is within this region, between z9343 (66.2 cM) and z4304 (72.2 cM). (c) b1128 was isolated in the TU background and out-crossed to AB. RAD marker positions suggest that b1128 is between 42.0 cM (z15460) and 50.4 cM (z5197) on LG 3. Individual genotypes confirm b1128 is within this region, approximately 0.7 cM from z5197 (50.4 cM). (d) b1166 was isolated in the AB background and out-crossed to WIK. RAD marker positions suggest that b1166 is between between 36.4 cM (z14229) and 45.7 cM (z43617) on LG 25. Individual genotyping confirms that b1166 is within this region.